TY  - JOUR
AU  - Chokheli, Vasiliy A.
AU  - Bakulin, Semyon D.
AU  - Ermolaeva, Olga Yu.
AU  - Rajput, Vishnu D.
AU  - Azarov, Anatoly S.
AU  - Kumari, Arpna
AU  - Stepanenko, Viсtoriya V.
AU  - Bushkova, Anastasia A.
AU  - Dmitriev, Pavel A.
AU  - Martinez-Montero, Marcos Edel
AU  - Varduni, Tatiana V.
PY  - 2023
DA  - 2023/07/20
TI  - An Efficient Method for Micropropagation of Red-List Herbaceous Plant Species (<i>Hedysarum cretaceum</i>)
JO  - OBM Genetics
SP  - 187
VL  - 07
IS  - 03
AB  - Hedysarum cretaceum Fisch is a critically endangered herbaceous species with high ornamental and phytomeliorative properties; thus, it needs urgent conservation and protection methods to conserve. In the present study, an efficient method for microtonal propagation of H. cretaceum is developed. The seed sterilization was optimized as treatment with 96% alcohol (2 min) and burned (1 sec) through a burner flame. Murashige and Skoog (MS) medium with the addition of 0.5 mg/L 6-Benzylaminopurine (BAP) (shoots per explant – 18.0) and 1.0 mg/L BAP and 0.1 mg/L Indole-3-acetic acid (IAA) (shoots per explant – 18.4) for the shoot and ⅓ MS supplemented with 0.1 mg/L IAA (rooting indicator – 62%) for roots were identified best composition for the proliferation and regeneration. Additionally, the medium for maintaining callus culture was determined – DKW with the addition of 2.5 mg/L BAP and 0.1 mg/L IAA, the possibility of direct organogenesis in vitro in the studied plant was also standardized. In addition, seed germination after various pre-sowing treatments was studied. It is shown that scarification makes achieving 80% germination of seeds possible. Also, sulfuric acid was used as a pre-sowing seed treatment, germination was 45%. Thus, using the protocols we developed, it is possible to maintain the H. cretaceum culture in vitro and increase seed germination using pre-sowing treatment.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.2303187
DO  - 10.21926/obm.genet.2303187
ID  - Chokheli2023
ER  -