TY  - JOUR
AU  - Suntornnont, Nuchanart
AU  - Kovavisarach, Ammaraluk
AU  - Tammachote, Rachaneekorn
PY  - 2025
DA  - 2025/01/06
TI  - Effects of Coenzyme Q10 on H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress of Human Keratinocytes
JO  - OBM Genetics
SP  - 279
VL  - 09
IS  - 01
AB  - Keratinocytes are particularly vulnerable to oxidative stress due to their function as the primary natural protective barrier exposed to chemicals, rays, and pollution to stimulate the formation of free radicals in the body. Two factors of interest correlate with cellular senescence under oxidative stress. These include regulating antioxidant synthesis, which maintains free-radical homeostasis through the Nrf2 gene, and the prevention of telomere shortening, which involves the collaboration of human telomerase genes, including the hTERT and hTR genes. Coenzyme Q10 (CoQ10) is a wildly used antioxidant in dietary supplements and the cosmetic industry. Therefore, this study aims to investigate the effect of CoQ10 in inhibiting the oxidation process induced by hydrogen peroxide on cell viability, intracellular ROS content, mRNA expression of the Nrf2 gene, hTERT gene, and hTR gene, and relative telomere lengths (RTL). When cells were treated with hydrogen peroxide at LC50 for 24 hours, followed by a 24-hour treatment with 1.00 µM CoQ10, cell viability increased by 16.57 ± 2.25%. Additionally, intracellular ROS content decreased by 37.20 ± 13.22%, Nrf2 gene expression significantly reduced (p < 0.001), and hTERT gene expression significantly increased (p < 0.01). No significant difference in the hTR gene expression and the RTL was detected. In conclusion, this study expands the knowledge of CoQ10 potential as an active ingredient in approaching anti-oxidant and anti-aging treatment.
SN  - 2577-5790
UR  - https://doi.org/10.21926/obm.genet.2501279
DO  - 10.21926/obm.genet.2501279
ID  - Suntornnont2025
ER  -