by Fatemeh Mollaamin  , Majid Monajjemi  and Ahmad R. Alsayed

Received: 22 February 2024;  Published: 19 June 2024;  doi: 10.21926/obm.genet.2402245

The potential and selective inhibitors of protein tyrosine phosphatase 1B (PTP1B) are therapeutically useful in treating type 2 diabetes. N-Benzoyl-L-glutamyl-[4-phosphono(difluoromethyl)]-L-phenylalanine-[4-phosphono(difluoro-methyl)]-L-phenylalanineamide (BzN-EJJ-amide) (BGD) which is the ligand of 1LQF protein code extracted from protein data bank (PDB) is an inhibitor of PTP-1B that indicates selectivity over several protein tyrosine phosphatases. In this research, the interaction between the anti-diabetic drug [...]

by Galal Khamis  , Jun Chi  and Qingshun Zhao

Received: 12 January 2024;  Published: 18 June 2024;  doi: 10.21926/obm.genet.2402244

Tomato (Solanum lycopersicum), a family of Solanaceae, is an annual crop grown in fields and greenhouses. Tomato is the fourth-largest agricultural crop in the world, with a market value of more than $50 billion. Numerous attempts were conducted on the tomato for further improvement via in vitro regeneration and transformation. This study aims to establish in vitro multiplication, transformation, and gene editing systems in tomatoes by using cotyledon explants. Cotyledon explants were placed on the MS medium suppor [...]

by Balamurugan Sivaprakasam  and Prasanna Sadagopan

Received: 24 December 2023;  Published: 14 June 2024;  doi: 10.21926/obm.genet.2402243

The present study introduces Snips2HLA-HsG, an integrated application designed for SNP genotype analysis and HLA allele type prediction. Leveraging attribute bagging, a powerful ensemble classifier technique from the Bioconductor HIBAG package, Snips2HLA-HsG offers a comprehensive response for genetic analysis. Accessible via https://snips2hla.shinyapps.io/hla_home/, the application distinguishes itself by prioritizing user-friendliness and integrating all-purpose functionalities, including sample preparation, mode [...]

by Buti Obaid Saeed Alfalahi  , Imane Lamdjad  , Noaman Atallah Alheety  , Mustafa Alnujaifi  and Abdul Qayyum

Received: 05 February 2024;  Published: 12 June 2024;  doi: 10.21926/obm.genet.2402242

Understanding the impact of melatonin (N-acetyl-5-methoxytryptamine) on plant gene expression is crucial for unlocking its full potential as a tool for crop improvement and stress tolerance. Melatonin has emerged to have several influences on the transcriptional activity of numerous genes, helping to orchestrate plant responses to environmental cues. Furthermore, it has been shown that melatonin signaling pathways control downstream gene expression to ensure proper plant growth and development. Therefore, clearing [...]

by Nadia A. Abdelmegeed Abdelwahed

Received: 12 March 2024;  Published: 12 June 2024;  doi: 10.21926/obm.genet.2402241

In this quantitative study, the researcher examined the screening strategies used to establish Egyptian women entrepreneurs' attitudes toward genetic technology. Using a questionnaire, the researcher collected data from 318 Egyptian women entrepreneurs. From applying the path analysis through Analysis of Moment Structures (AMOS) version 26.0, this study’s findings demonstrate genomic knowledge’s positive effect on attitudes towards genetic technology (β = 0.176; p = 0.003). The path analysis shows that, on the one [...]

by Ana Luísa Teixeira Fontes  , Niklas Padutsch  , Stefanie Kankel  and Thomas Liehr

Received: 15 April 2024;  Published: 11 June 2024;  doi: 10.21926/obm.genet.2402240

Balanced chromosomal rearrangements, including translocations, contribute to infertility, repeated abortions, and/or genetically imbalanced offspring in corresponding carriers. A translocation is usually considered a unique, de novo, or familial event. Besides, some translocations have also been shown to develop multiple times with slightly different or even identical breakpoints; for others, founder effects have been suggested. Here, two known recurrent translocations [t(11;22)(q23.3;q11.21) and der(X)t(X; Y)(p22. [...]

by Upasana Ramphal  , Derek Tshiabuila  , Yajna Ramphal  , Jennifer Giandhari  , Carel van Heerden  , Cheryl Baxter  , Stephanie van Wyk  , Sureshnee Pillay  , Oluwakemi Laguda-Akingba  , Eduan Wilkinson  , Richard Lessells  and Tulio de Oliveira

Received: 01 March 2024;  Published: 30 May 2024;  doi: 10.21926/obm.genet.2402239

During the SARS-CoV-2 pandemic, next-generation sequencing (NGS) technologies like the Ion Torrent S5 and Illumina MiSeq, alongside advanced software, improved genomic surveillance in South Africa. This study analysed anonymized samples from the Eastern Cape using Genome Detective and NextClade, showing Ion Torrent S5 and Illumina MiSeq success rates of 96% and 94%, respectively. The study focused on genomic coverage (above 80%) and mutation detection (below 100), with the Ion Torrent S5 achieving 99% coverage comp [...]

by Alessandro Stasi  and Onnida Thongpravati

Received: 04 March 2024;  Published: 29 May 2024;  doi: 10.21926/obm.genet.2402238

The expansion of do-it-yourself (DIY) gene editing, facilitated by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology, has catalyzed a significant shift in scientific research and biotechnology innovation. This movement is propelled by a community-driven approach that challenges the traditional confines of scientific exploration, allowing amateur scientists to perform sophisticated biological experiments. While this democratization fosters inclusivity and accelerates innovation, it simult [...]

by Ahmad R. Alsayed  and Andi Dian Permana

Received: 28 February 2024;  Published: 28 May 2024;  doi: 10.21926/obm.genet.2402237

Bacteriophages, or phages, are viruses that infect bacteria, exhibiting specificity towards particular bacterial strains. Despite being overshadowed by traditional antibiotics in the West, interest in phage therapy has resurged due to the escalating antimicrobial resistance crisis. Understanding phage biology, selection, production, and pharmacology is crucial for their clinical application. Phages interact intricately with the human microbiome, influencing bacterial populations and potentially offering therapeutic [...]

by Emad Fadhal

Received: 18 January 2024;  Published: 24 May 2024;  doi: 10.21926/obm.genet.2402236

The RAS signaling pathway is a crucial cell transduction pathway central to transmitting signals from outside the cell to the cell nucleus and influencing fundamental biological mechanisms like cell growth, division, and specialization. This signaling pathway has recently received much attention in scientific research because of its involvement in various diseases, especially carcinogenesis. Our study identified the significance of crucial proteins in the RAS signaling cascade in cancer development and progression. [...]