Development of a Novel Pipette Tip-Aided Cell Cloning Method for The Effective Isolation of Genome-Edited Porcine Cell
OBM Genetics is an international Open Access journal published quarterly online by LIDSEN Publishing Inc. It accepts papers addressing basic and medical aspects of genetics and epigenetics and also ethical, legal and social issues. Coverage includes clinical, developmental, diagnostic, evolutionary, genomic, mitochondrial, molecular, oncological, population and reproductive aspects. It publishes research articles, reviews, communications and technical notes, etc. There is no restriction on the length of the papers and we encourage scientists to publish their results in as much detail as possible.
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Submission Deadline: October 31, 2020 (Open) Submit Now
Masahiro Sato, Ph.D.
Professor, Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-8544, Japan
Research Interests: Molecular biology; biotechnology; genome editing; gene expression; mice model; xenotransplantation; immunofluorescence staining
About this topic
Genome editing, genome engineering, or gene editing is a type of genetic engineering in which DNA is inserted, deleted, modified or replaced in the genome of a living organism. Unlike early genetic engineering techniques that randomly insert genetic material into a host genome, genome editing targets the insertions to specific locations.
Genome editing is of great interest in the prevention and treatment of human diseases. Currently, most research on genome editing is done to understand diseases using cells and animal models. Scientists are still working to determine whether this approach is safe and effective for use in people. It is being explored for a wide variety of diseases, including single-gene disorders such as cystic fibrosis, hemophilia, and sickle cell disease. It also holds promise for the treatment and prevention of more complex diseases, such as cancer, heart disease, mental illness, and human immunodeficiency virus (HIV) infection. In plants, genome editing is seen as a viable solution to the conservation of biodiversity. Gene drive is a potential tool to alter the reproductive rate of invasive species, though there are significant associated risks. Therefore, in this special issue, we will seek articles that reflect the research on genome editing. Original research reports, review articles, communications, perspectives, etc., are invited in all areas pertinent to this topic.
Title: In vitro electroporation facilitates simultaneous genome editing induction at least 3 multi-loci in porcine parthenotes.
Authors: Masahiro Sato, Emi Inada, Issei Saitoh, Hiroaki Kawaguchi, Akihide Tanimoto, and Kazuchika Miyoshi
Abstract: Clustered regularly interspaced short palindromic repeat-associated protein 9 (CRISPR/Cas9) system allows simultaneous genome editing towards multiple target loci using several guide RNAs (gRNAs) together with Cas9 in various biological systems. In vitro electroporation (EP) of zygotes in the presence of gRNA/Cas9 complex called ribonucleoprotein (RNP) has been widely employed for production of genome-edited rodents. This approach is powerful and convenient comparing to microinjection-based production of genome-edited rodents, because large number of zygotes (30-50 embryos per in vitro EP) can be genome-edited at once. In contrast with the case of mice, only a few cases are known for successful production of genome-edited porcine embryos and born piglets by using in vitro EP. In this study, we evaluated the ability of in vitro EP to introduce multiple substances into porcine parthenotes when 2 fluorescence-labelled dextrans or 3 gRNAs complexed with Cas9 protein are used.
Title: Breeding “CRISPR” Crops
Authors: Fawzy Georges
Abstract: The challenges which face the world today can be summed up in a few words: An increasingly congested world with dwindling areas of viable cultivated land and accelerating climate instability. The combined effect of these realities, together with the trend of striving to extend the average human age, puts the world on the path toward future catastrophe. This situation makes it imperative to seek realistic and practical solutions, which must be able to address food shortages and climate problems in a timely manner. In this article, an elucidative argument is presented with the intention of revealing the need for humanity to step back and consider more objectively the long-term benefits of crop-genome-editing for food security, looking beyond the unfounded negative notions about safety issues. If the inaccurate interpretations, formulated by some political scientists and (or) policy makers, which claim the CRISPR/Cas technology as being another form of crop genetic modification stay unabated, they will continue to becloud the minds of decision makers and the public at large with misleading information. The technology will eventually be dismissed as a mere academic exercise with little or no benefit to future generations.
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