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Next-Generation Sequencing Technology - Progress and Future in Plant Pathogens Identification and Detection
Submission Deadline: January 31, 2025 (Open) Submit Now
Leila Zirak, PhD
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, 5166616471, Tabriz, Iran
Research interests: Phytoplasma; next generation sequencing; plant pathology
About This Topic
Several detection methods such as PCR-based methods are used to detection of phytopathogens especially viruses or non-culturable bacteria. While application of these methods confronts many limitations. Non-culturable bacteria detection often is hampered by their irregular distribution in plants especially in the trees. Also, PCR-based methods require preparation of DNA in accurate concentrations. In the other hand, all primers exhibit some homology to chloroplast and mitochondrial DNAs. The Next-generation sequencing (NGS) is introduced as potentially powerful screening method for mixed infection diseases diagnostics in plants and pathogens which could not detectable by other detection methods. In plant viruses detection, in which viral disease symptoms are absent, unspecific or triggered by multiple viruses, by the NGS based methods is possible. The NGS enables highly efficient, rapid DNA or RNA high-throughput sequencing of plant virus, viroids and non-culturable bacteria genomes. Although NGS is not so much familiar in the plant diseases field however, development of NGS-based methods can have a new perspective in detection of plant pathogens.
Plant pathogens detection; next-generation sequencing; mixed infection disease.
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